Illumina metagenomic analysis manual

Author

Luc van Zon

Published

April 9, 2025

Introduction

Welcome to the Illumina metagenomic data analysis manual. This manual contains a step by step guide for performing quality control, filtering host sequences, assembling reads into contigs, annotating the contigs, and then extracing viral contigs and their corresponding reads. In the final chapter of the manual we will show how to automate all of these steps into a single pipeline for speed and convenience.

Tip

If you are just interested in running the automated workflow, then you only have to check out the chapters ‘Preparation’ and ‘Automating data analysis’.